Recombinant Human IL-2R beta/CD122 Protein (ECD, His Tag), HPLC-verified

概述

产品介绍

Cat No. IL-2-PH4101

库存：现货

Size

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产品介绍

纯度

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED₅₀ for this effect is 1-6ug/ml.
2.Loaded Recombinant Human IL-2R beta/CD122 Protein, His Tag (Cat. No. 10696-H08B) on His1K Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 0.76 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).

蛋白构建

A DNA sequence encoding the human IL2RB (NP_000869.1) (Met1-Asp239) was expressed with a polyhistidine tag at the C-terminus.

Accession#

NP_000869.1

表达宿主

Baculovirus-Insect Cells

种属

Human

预测 N 端

Ala 27

分子量

The recombinant human IL2RB consists of 224 amino acids and predicts a molecular mass of 26 kDa.

缓冲液

Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0, 10 % glycerol.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED₅₀ for this effect is 1-6ug/ml

Loaded Recombinant Human IL-2R beta/CD122 Protein, His Tag (Cat. No. 10696-H08B) on His1K Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 0.76 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).

Recombinant Human IL-2R beta/CD122 Protein (ECD, His Tag), HPLC-verified: 别称

CD122 Protein, Human; IL15RB Protein, Human; IMD63 Protein, Human; P70-75 Protein, Human

IL-2R beta/CD122 背景信息

Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

全称

interleukin 2 receptor, beta

研究领域

Neuroinflammation

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参考文献

Foss F., et al., 2006, Semin Oncol. 33(1 Suppl 3): 11-6.
Sprent J, et al., 2001, Science. 293(5528): 245-8.
Teshigawara K, et al. , 1987, J Exp Med. 165(1): 223-38.