≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the mouse IL2RB (P16297) (Met1-Glu240) was expressed with a C-terminal polyhistidine tag.
Accession#
P16297
表达宿主
HEK293 Cells
种属
Mouse
预测 N 端
Ala 27
分子量
The recombinant mouse IL2RB comprises 225
amino acids and has a predicted molecular mass of 26.5 kDa. The apparent
molecular mass of the protein is approximately 45-54 kDa in SDS-PAGE
under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk
packages of recombinant proteins are provided as frozen liquid. They
are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months
from date of receipt at -20℃ to -80℃. Store it under sterile conditions
at -20℃ to -80℃. It is recommended that the protein be aliquoted for
optimal storage. Avoid repeated freeze-thaw cycles.
复溶
To find reconstitution info, click on 'COA'
at the top of the page, enter the lot number and product size for the
lot-specific COA.
Recombinant Mouse IL-2R beta/CD122 Protein (His Tag), HPLC-verified: 别称
Interleukin-2 receptor (IL-2R) also
known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit
beta, and IL-2RB, is involved in T cell-mediated immune responses.
CD122/IL-2RB is present in 3 forms concerning the ability to bind
interleukin 2. The low-affinity form is a monomer of the alpha subunit
and is not involved in signal transduction. The intermediate affinity
form consists of an alpha/beta subunit heterodimer, while the
high-affinity form consists of an alpha/beta/gamma subunit heterotrimer.
Both the intermediate and high-affinity forms of CD122/IL-2RB are
involved in receptor-mediated endocytosis and transduction of mitogenic
signals from interleukin 2. CD122/IL-2RB expression was restricted to
the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that
proliferate vigorously to IL-2 in the absence of any stromal cells, but
not to IL-15. The high-affinity form of this receptor is expressed on
activated T lymphocytes, activated B lymphocytes, and activated
macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte
development.
