Recombinant Rat IL-2R beta/CD122 Protein (ECD, His & AVI Tag), Avi-tag Biotinylated, HPLC-verified

概述

产品介绍

Cat No. IL-2-PR1102

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

Loaded Recombinant Rat IL-2R beta / CD122 Protein, His Tag, Biotinylated (Cat. No. 80340-R49H-B) on SA Biosensor, can bind Recombinant Rat IL-2 Protein (Cat. No. 80075-RNAE) with an affinity constant of 6.27 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白构建

A DNA sequence encoding the Rat IL-2R beta / CD122 (NP_037327.1) (Met1-Glu239) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

表达宿主

HEK293 Cells

种属

Rat

预测 N 端

Ala 27

分子量

The recombinant Rat IL-2R beta / CD122 consisting of 239 amino acids and has a calculated molecular mass of 28.17 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 41 kDa protein in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Recombinant Rat IL-2R beta/CD122 Protein (ECD, His & AVI Tag), Avi-tag Biotinylated, HPLC-verified: 别称

Il2rb Protein, Rat; IL2RBC Protein, Rat

IL-2R beta/CD122 背景信息

Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

全称

interleukin 2 receptor, beta

研究领域

Neuroinflammation

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参考文献

Foss F., et al., 2006, Semin Oncol. 33(1 Suppl 3): 11-6.
Sprent J, et al., 2001, Science. 293(5528): 245-8.
Teshigawara K, et al. , 1987, J Exp Med. 165(1): 223-38.