≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit
IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells
in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1-4μg/mL.
蛋白构建
The mature form of human IL2 Rβ (NP_000869.1)
extracellular domain (Met 1-Asp 239) with five amino acids (DDDDK) at
the C-terminus was expressed and purified.
Accession#
NP_000869.1
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Ala 27
分子量
The mature recombinant human IL2 Rβ consists
of 219 amino acids and has a predicted molecular mass of 25.2 kDa. As a
result of glycosylation, the rh IL2 Rβ migrates as an approximately 40
kDa protein in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk
packages of recombinant proteins are provided as frozen liquid. They
are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months
from date of receipt at -20℃ to -80℃. Store it under sterile conditions
at -20℃ to -80℃. It is recommended that the protein be aliquoted for
optimal storage. Avoid repeated freeze-thaw cycles.
复溶
To find reconstitution info, click on 'COA'
at the top of the page, enter the lot number and product size for the
lot-specific COA.
Recombinant Human IL-2R beta/CD122 Protein, HPLC-verified: 别称
Interleukin-2 receptor (IL-2R) also
known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit
beta, and IL-2RB, is involved in T cell-mediated immune responses.
CD122/IL-2RB is present in 3 forms concerning the ability to bind
interleukin 2. The low-affinity form is a monomer of the alpha subunit
and is not involved in signal transduction. The intermediate affinity
form consists of an alpha/beta subunit heterodimer, while the
high-affinity form consists of an alpha/beta/gamma subunit heterotrimer.
Both the intermediate and high-affinity forms of CD122/IL-2RB are
involved in receptor-mediated endocytosis and transduction of mitogenic
signals from interleukin 2. CD122/IL-2RB expression was restricted to
the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that
proliferate vigorously to IL-2 in the absence of any stromal cells, but
not to IL-15. The high-affinity form of this receptor is expressed on
activated T lymphocytes, activated B lymphocytes, and activated
macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte
development.
