Recombinant Human IL-2Rβ & IL-2Rγ Heterodimer Protein, Avi-tag Biotinylated, HPLC-verified

概述

产品介绍

Cat No. IL-2-PH1003

库存：现货

Size

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产品介绍

纯度

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

1.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 2.190 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAH1-E) with an affinity constant of 1.210 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).

蛋白构建

A DNA sequence encoding the Human IL2RB (P14784)(Ala27-Asp239) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Human IL2RG (P31785) (Leu23-Asn254) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL2RB & IL2RG heterodimer was purified.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Ala 27 & Leu 23

分子量

The recombinant heterodimer of human IL2RB & IL2RG comprises 616 (313+303) amino acids and has a calculated molecular mass of 68.34 (34.25+34.09) KDa. The apparent molecular mass of human IL2RB & IL2RG heterodimer is approximately 59 kDa and 45 kDa respectively in SDS-PAGE. The molecular weight of this protein is around 98.7 kDa verified by SEC-MALS(Routinely tested).

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 2.190 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAH1-E) with an affinity constant of 1.210 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).

The purity of Recombinant Human IL-2Rβ & IL-2Rγ Protein,Flag & AVI &His Tag,Biotinylated,HPLC-verified(Cat. No. CT108-H2549H1-B)was more than 95% and the molecular weight of this protein is around 98.7 kDa verified by SEC-MALS(Routinely tested).