Recombinant Rat CD25/IL-2R alpha Protein (ECD, His & AVI Tag), Avi-tag Biotinylated

概述

产品介绍

Cat No. IL-2-PR4101

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

Immobilized Recombinant Rat CD25 / IL-2R alpha Protein (ECD, His & AVI Tag), Biotinylated (Cat: 80074-R49B-B ) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E), the EC₅₀ is 5-15 ng/mL.

蛋白构建

A DNA sequence encoding the Rat IL2RA (NP_037295.1) (Met1-Gln235) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

表达宿主

Baculovirus-Insect Cells

种属

Rat

预测 N 端

Glu 22

分子量

The recombinant Rat IL2RA consisting of 240 amino acids and has a calculated molecular mass of 27.72 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 41.2 kDa protein in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Recombinant Rat CD25/IL-2R alpha Protein (ECD, His & AVI Tag), Avi-tag Biotinylated: 别称

Il2ra Protein, Rat; IL2RAC Protein, Rat

CD25/IL-2R alpha 背景信息

CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors.

全称

interleukin 2 receptor, alpha

研究领域

Neuroinflammation
Cancer Drug Targets

相关通路图

AKT Signaling Pathway

Jak-Stat Signaling Pathway

Autophagy Pathway

Common G-Chain signaling pathway

参考文献

Driesen J, et al. (2008) CD25 as an immune regulatory molecule expressed on myeloid dendritic cells. Immunobiology. 213(9-10): 849-58.
Olejniczak K, et al. (2008) Biological properties of interleukin 2 and its role in pathogenesis of selected diseases--a review. Med Sci Monit. 14(10): RA179-89.
Chistiakov DA, et al. (2008) The crucial role of IL-2/IL-2RA-mediated immune regulation in the pathogenesis of type 1 diabetes, an evidence coming from genetic and animal model studies. Immunol Lett. 118(1): 1-5.
Bien E, et al. (2008) Serum soluble interleukin 2 receptor alpha in human cancer of adults and children: a review. Biomarkers. 13(1): 1-26.