Recombinant Human CD25/IL-2R alpha Protein (ECD, His Tag), HPLC-verified, Endotoxin-Free

概述

产品介绍

Cat No. IL-2-PH1110

库存：现货

Size

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产品介绍

纯度

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).

内毒素

< 0.5 EU per mg protein.

生物活性

Testing in progress

蛋白构建

A DNA sequence encoding the extracellular domainof human IL2RA (NP_000408.1) (Met1-Cys213) was expressed with a C-terminal polyhistidine tag.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Glu 22

分子量

The recombinant human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation. The molecular weight of this protein is around 32.4 kDa verified by SEC-MALS (Routinely tested).

缓冲液

Supplied as sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

It is supplied and shipped as liquid with dry ice.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

The purity of Recombinant Human IL2Ra / CD25 Protein,His Tag,HPLC-verified(Cat. No. 10165-H08H-UE)was more than 95% and the molecular weight of this protein is around 32.4 kDa verified by SEC-MALS(Routinely tested).

Recombinant Human CD25/IL-2R alpha Protein (ECD, His Tag), HPLC-verified, Endotoxin-Free: 别称

CD25 Protein, Human; IDDM10 Protein, Human; IL2R Protein, Human; IMD41 Protein, Human; p55 Protein, Human; TCGFR Protein, Human

CD25/IL-2R alpha 背景信息

CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors.

全称

interleukin 2 receptor, alpha

研究领域

Neuroinflammation
Cancer Drug Targets

相关通路图

AKT Signaling Pathway

Jak-Stat Signaling Pathway

Autophagy Pathway

Common G-Chain signaling pathway

参考文献

Driesen J, et al. (2008) CD25 as an immune regulatory molecule expressed on myeloid dendritic cells. Immunobiology. 213(9-10): 849-58.
Olejniczak K, et al. (2008) Biological properties of interleukin 2 and its role in pathogenesis of selected diseases--a review. Med Sci Monit. 14(10): RA179-89.
Chistiakov DA, et al. (2008) The crucial role of IL-2/IL-2RA-mediated immune regulation in the pathogenesis of type 1 diabetes, an evidence coming from genetic and animal model studies. Immunol Lett. 118(1): 1-5.
Bien E, et al. (2008) Serum soluble interleukin 2 receptor alpha in human cancer of adults and children: a review. Biomarkers. 13(1): 1-26.