Recombinant Mouse IL-7 Protein

概述

产品介绍

Cat No. IL-7-PM2001

库存：现货

Size

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产品介绍

纯度

> 90 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood mononuclear cell (PBMC). The ED₅₀ for this effect is typically 0.4-4 ng/mL.

蛋白构建

A DNA sequence encoding the mouse IL7 (NP_032397.1) (Glu26-Ile154) was expressed and purified with an initial Met.

Accession#

NP_032397.1

表达宿主

E. coli

种属

Mouse

预测 N 端

Met

分子量

The recombinant mouse IL7 consists of 130 amino acids and predicts a molecular mass of 15 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

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Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood mononuclear cell (PBMC). The ED₅₀ for this effect is typically 0.4-4 ng/mL.

Recombinant Mouse IL-7 Protein: 别称

A630026I06Rik Protein, Mouse; hlb368 Protein, Mouse; Il-7 Protein, Mouse; Il7 Protein, Mouse

IL-7 背景信息

IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

全称

interleukin 7

相关通路图

Common G-Chain signaling pathway

参考文献

Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes.
J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses. Immunity. 30 (3):447-57.
Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10): 2541-4.