Recombinant Human IL-7 Protein, GMP grade, HPLC-verified

概述

产品介绍

Cat No. IL-7-PH2003

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 0.01 EU per μg of the protein.

生物活性

Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). The ED₅₀ for this effect is typically 0.5-8 ng/mL. The specific activity of Recombinant Human IL-7 is >100，000 units/μg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530).

蛋白构建

A DNA sequence encoding the Human IL7 (NP_000871.1) (Asp26-His177) was expressed with a N-terminal Met.

Accession#

NP_000871.1

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant IL7 consists of 153 amino acids and predicts a molecular mass of 17.5 kDa.

缓冲液

Supplied as powder that is lyophilized from a 0.2 um filtered solution in PBS,Trehalose and mannitol.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

稳定性 & 储存条件

Samples are stable for up to thirty-six months from date of receipt at ≤ -20℃
Store it under sterile conditions at ≤-20℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Recombinant Human IL-7 Protein, GMP grade, HPLC-verified: 别称

IL-7 Protein, Human

IL-7 背景信息

IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

全称

interleukin 7

相关通路图

Common G-Chain signaling pathway

参考文献

Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes.
J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses. Immunity. 30 (3):447-57.
Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10): 2541-4.