Recombinant Human IL-15 Protein, GMP grade, HPLC-verified, DMF Filed

概述

产品介绍

Cat No. IL-15-PH2002

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 10 EU per mg protein.

生物活性

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 3-12 ng/mL.

蛋白构建

A DNA sequence encoding the human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.

Accession#

NP_000576.1

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human IL15 consists of 115 amino acids and predicts a molecular mass of 12.91 kDa.

缓冲液

Supplied as sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

Liquid. It is shipped out with blue ice.

稳定性 & 储存条件

Samples are stable for up to thirty-six months from date of receipt at -20℃ to -80℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 3-12 ng/mL.

Recombinant Human IL-15 Protein, GMP grade, HPLC-verified, DMF Filed: 别称

IL-15 Protein, Human; MGC9721 Protein, Human

IL-15 背景信息

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.

全称

interleukin 15

相关通路图

AKT Signaling Pathway

Jak-Stat Signaling Pathway

Autophagy Pathway

Common G-Chain signaling pathway

参考文献

Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md : 1950). 2015;195(3):901-923.