Recombinant Mouse IL15 & IL15RA Protein (His Tag), HPLC-verified

概述

产品介绍

Cat No. IL-15-PM1101

库存：现货

Size

点击咨询在线客服

产品介绍

纯度

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Testing in progress

蛋白构建

A DNA sequence encoding the mouse IL15 (P48346-1)(Met1-Ser162) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse IL15RA (Q60819-1) (Met1-Lys205) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL15 & IL15RA heterodimer was purified.

Accession#

P48346-1 & Q60819-1

表达宿主

HEK293 Cells

种属

Mouse

预测 N 端

mouse IL15 (Gly 30 & Asn 49) & mouse IL15RA (Gly 33)

分子量

The recombinant heterodimer of mouse IL15 & IL15RA comprises 308 (125+183) amino acids and has a calculated molecular mass of 34.46 (14.70+19.76) kDa. The apparent molecular mass of mouse IL15 & IL15RA heterodimer is approximately (50.02, 23.49, 21.78 and 19.76) kDa respectively in SDS-PAGE.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

IL15 & IL15RA 背景信息

IL-15 and IL-15 receptor alpha (IL-15Rα) play a significant role in multiple aspects of T cell biology. Interleukin (IL)-15 is essential for natural killer (NK), NKT and memory (m) CD8+ T cell development and function, and is currently under investigation as an immunotherapeutic agent for the treatment of cancer. Recently, the creation of IL-15 superagonist by complexing IL-15 and its high affinity receptor alpha (IL-15 Rα) in solution, inspired by the natural trans-presentation of IL-15, advances the potential of IL-15-based tumor immunotherapy. Gene therapy using engineered cells co-expressing IL-15/IL-15 Rα complex for cancer treatment is also emerging.