Recombinant Human IL15 & IL15RA Protein (hFc Tag), HPLC-verified

概述

产品介绍

Cat No. IL-15-PH1201

库存：现货

Size

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产品介绍

纯度

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells (QC tested).

蛋白构建

A DNA sequence encoding the human IL15RA (NP_002180.1) (Ile31-Asp96) linked with the human IL15 (NP_000576.1, with mutation Asn 120 Asp) (Asn49-Ser162) by a peptide linker was fused with the Fc region of human IgG1 at the C-terminus.

Accession#

NP_002180.1 & NP_000576.1

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Ile 31

分子量

The recombinant heterodimer of human IL15RA & IL-15 (N120D) consists of 433 amino acids and predicts a molecular mass of 47.9 kDa.

缓冲液

Lyophilized from sterile 50mMTris, 100mMNacl, 5%trehalose，0.05%chaps, pH8.5.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

IL15 & IL15RA 背景信息

IL-15 and IL-15 receptor alpha (IL-15Rα) play a significant role in multiple aspects of T cell biology. Interleukin (IL)-15 is essential for natural killer (NK), NKT and memory (m) CD8+ T cell development and function, and is currently under investigation as an immunotherapeutic agent for the treatment of cancer. Recently, the creation of IL-15 superagonist by complexing IL-15 and its high affinity receptor alpha (IL-15 Rα) in solution, inspired by the natural trans-presentation of IL-15, advances the potential of IL-15-based tumor immunotherapy. Gene therapy using engineered cells co-expressing IL-15/IL-15 Rα complex for cancer treatment is also emerging.