> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物素标记率
0.7-1 as determined by the HABA assay.
生物活性
Immobilized Recombinant Human IL-15 / IL15 /
Interleukin 15 Protein (His Tag)(Cat:10360-H07E) at 2 μg/ml (100
μl/well) can bind Recombinant Human IL15RA Protein (ECD, Fc & AVI
Tag), Biotinylated(Cat:29736-H41H-B), The EC50 is 12-36 ng/mL.
蛋白构建
A DNA sequence encoding the human IL15RA
(NP_002180.1) (Met1-Thr205) was expressed with a c-terminal AVI tagged
Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed
protein was biotinylated in vivo by the Biotin-Protein ligase (BirA
enzyme) which is co-expressed.
Accession#
NP_002180.1
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Ile 31
分子量
The recombinant Human IL15RA consists of 428
amino acids and predicts a molecular mass of 46.92 kDa. It migrates as
an approximately 73.09 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk
packages of recombinant proteins are provided as frozen liquid. They
are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months
from date of receipt at -20℃ to -80℃. Store it under sterile conditions
at -20℃ to -80℃. It is recommended that the protein be aliquoted for
optimal storage. Avoid repeated freeze-thaw cycles.
复溶
To find reconstitution info, click on 'COA'
at the top of the page, enter the lot number and product size for the
lot-specific COA.
Immobilized Recombinant Human IL-15 / IL15 / Interleukin 15 Protein (His
Tag)(Cat:10360-H07E) at 2 μg/ml (100 μl/well) can bind Recombinant
Human IL15RA Protein (ECD, Fc & AVI Tag),
Biotinylated(Cat:29736-H41H-B), The EC50 is 12-36 ng/mL.
Recombinant Human IL-15 R alpha/IL15RA Protein (ECD, hFc & AVI Tag), Biotinylated, HPLC-verified: 别称
