Recombinant Human IL-21 Protein, GMP grade, HPLC-verified, DMF Filed

概述

产品介绍

Cat No. IL-21-PH2001

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 5 EU per mg of the protein.

生物活性

Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.
The ED₅₀ for this effect is 0.4-2 ng/mL.

蛋白构建

A DNA sequence encoding the mature form of human IL21 isoform 1 (Q9HBE4-1) (Gln30-Ser162) was expressed and purified with an initial Met.

Accession#

Q9HBE4-1

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human IL-21 consists of 134 amino acids and has a calculated molecular mass of 15.6 kDa.

缓冲液

Supplied as sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

Liquid. It is shipped out with blue ice.

稳定性 & 储存条件

Samples are stable for up to thirty-six months from date of receipt at -20℃ to -80℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is typically 0.4-2 ng/mL.

Recombinant Human IL-21 Protein, GMP grade, HPLC-verified, DMF Filed: 别称

CVID11 Protein, Human; IL-21 Protein, Human; Za11 Protein, Human

IL-21 背景信息

IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation).

全称

interleukin 21

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参考文献

Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34.
Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10.
Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.