Recombinant Mouse IL-21 Protein

概述

产品介绍

Cat No. IL-21-PM2001

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE

内毒素

Please contact us for more information.

生物活性

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is typically 10-65 ng/mL.

蛋白构建

A DNA sequence encoding the soluble form of mouse IL21 (Q9ES17) (Pro 25-Ser 146) was expressed, with an initial Met at the N-terminus.

Accession#

Q9ES17

表达宿主

E. coli

种属

Mouse

预测 N 端

Met

分子量

The recombinant mouse IL21 consists of 123 amino acids and has a calculated molecular mass of 14.3KDa. It migrates as a 15 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is typically 10-65 ng/mL.

Recombinant Mouse IL-21 Protein: 别称

IL-21 Protein, Mouse; Il21 Protein, Mouse

IL-21 背景信息

IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation).

全称

interleukin 21

相关通路图

AKT Signaling Pathway

Jak-Stat Signaling Pathway

Autophagy Pathway

Common G-Chain signaling pathway

参考文献

Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34.
Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10.
Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.