Recombinant Human IL-21 Protein, GMP grade, Animal-Free, HPLC-verified

概述

产品介绍

Cat No. IL-21-PH2003

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

<10 eu="" per="" mg="" protein.="">

生物活性

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is 0.4-2 ng/ml.

蛋白构建

A DNA sequence encoding the mature form of human IL21 isoform 1 (Q9HBE4-1) (Gln30-Ser162) was expressed and purified with an initial Met.

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human IL-21 consists of 134 amino acids and has a calculated molecular mass of 15.6 kDa.

缓冲液

Supplied as powder that is lyophilized from a 0.2 um filtered solution in PBS and Trehalose.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

稳定性 & 储存条件

Samples are stable for up to thirty-six months from date of receipt at ≤ -20℃
Store it under sterile conditions at ≤-20℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is 0.4-2 ng/ml.

Recombinant Human IL-21 Protein, GMP grade, Animal-Free, HPLC-verified: 别称

CVID11 Protein, Human; IL-21 Protein, Human; Za11 Protein, Human

IL-21 背景信息

IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation).

全称

interleukin 21

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参考文献

Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34.
Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10.
Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.