Recombinant Human IL-22 Protein, MALS-verified

概述

产品介绍

Cat No. IL-21-PH2004

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS.

内毒素

< 10 EU per mg protein.

生物活性

1.Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is typically 0.3-1.2 ng/mL.
2.Human small intestinal organoids were cultured with IL22(Cat#13059-HNAE), FGF10(Cat#10573-HNAE), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.

蛋白构建

A DNA sequence encoding human IL22(NP_065386.1) (Ala34-Ile179) was expressed with a N-terminal Met.

Accession#

NP_065386.1

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human IL22 consists of 147 amino acids and predicts a molecular mass of 16.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 18.4 kDa verified by SEC-MALS.

缓冲液

Lyophilized from sterile PBS, pH 8.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Human small intestinal organoids were cultured with IL22(Cat#13059-HNAE), FGF10(Cat#10573-HNAE), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.

The purity of Recombinant Human IL22 / IL-22 / Interleukin 22 Protein,MALS-verified(Cat. No. 13059-HNAE)was more than 90% and the molecular weight of this protein is around 18.4 kDa verified by SEC-MALS.

Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is typically 0.3-1.2 ng/mL.

Recombinant Human IL-22 Protein, MALS-verified: 别称

IL-21 Protein, Human; IL-22 Protein, Human; IL-D110 Protein, Human; IL-TIF Protein, Human; ILTIF Protein, Human; MGC79382 Protein, Human; MGC79384 Protein, Human; TIFa Protein, Human; TIFIL-23 Protein, Human; UNQ3099/PRO10096 Protein, Human; zcyto18 Protein, Human

IL-22 背景信息

IL22 is a member of a group of cytokines called the IL-1 family of IL-1 superfamily (including IL-19, IL-2, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares the use of IL-1R2 in cell signaling with other members of this family, IL-1, IL-26, IL-28A/B, and IL-29. IL22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-1R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (SIL-22R1). IL22 and IL-1 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S1s, and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-1. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL-17A.

全称

interleukin 22

参考文献

Pestka S. et al., 2004, Annu Rev Immunol. 22: 929-79.
Xie MH. et al., 2000, J Biol Chem. 275 (40): 31335-9.
Jones BC. et al., 2008, Structure. 16 (9): 1333-44.