Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 0.2-0.8 ng/mL.
蛋白构建
A DNA sequence encoding the human IL22 (NP_065386.1) (Ala34-Ile179) was expressed with a polyhistidine tag at the C-terminus.
Accession#
NP_065386.1
表达宿主
Yeast
种属
Human
预测 N 端
Ala 34
分子量
The recombinant human IL22 consists of 156 amino acids and predicts a molecular mass of 18.1 kDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk
packages of recombinant proteins are provided as frozen liquid. They
are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months
from date of receipt at -20℃ to -80℃. Store it under sterile conditions
at -20℃ to -80℃. It is recommended that the protein be aliquoted for
optimal storage. Avoid repeated freeze-thaw cycles.
复溶
To find reconstitution info, click on 'COA'
at the top of the page, enter the lot number and product size for the
lot-specific COA.
IL22 is a member of a group of cytokines
called the IL-1 family of IL-1 superfamily (including IL-19, IL-2,
IL-24, and IL-26), a class of potent mediators of cellular inflammatory
responses. It shares the use of IL-1R2 in cell signaling with other
members of this family, IL-1, IL-26, IL-28A/B, and IL-29. IL22 is
produced by activated DC and T cells and initiates innate immune
responses against bacterial pathogens especially in epithelial cells
such as respiratory and gut epithelial cells. IL22 along with IL-17 is
rapidly produced by splenic LTi-like cells and can be also produced by
Th17 cells and likely plays a role in the coordinated response of both
adaptive and innate immune systems. IL22 biological activity is
initiated by binding to a cell-surface complex composed of IL-22R1 and
IL-1R2 receptor chains and further regulated by interactions with a
soluble binding protein, IL-22BP, which shares sequence similarity with
an extracellular region of IL-22R1 (SIL-22R1). IL22 and IL-1 receptor
chains play a role in cellular targeting and signal transduction to
selectively initiate and regulate immune responses. IL22 can contribute
to immune disease through the stimulation of inflammatory responses,
S1s, and defensins. IL22 also promotes hepatocyte survival in the liver
and epithelial cells in the lung and gut similar to IL-1. In some
contexts, the pro-inflammatory versus tissue-protective functions of
IL22 are regulated by the often co-expressed cytokine IL-17A.
全称
interleukin 22
参考文献
Pestka S. et al., 2004, Annu Rev Immunol. 22: 929-79.
