Recombinant Human IL-21 Receptor/IL-21R Protein (His & AVI Tag), Biotinylated,

Recombinant Human IL-21 Receptor/IL-21R Protein (His & AVI Tag), Biotinylated, HPLC-verified

概述

产品介绍

Cat No. IL-21-PH1101

库存：现货

Size

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产品介绍

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

Immobilized Recombinant Human Interleukin-21 / IL-21 Protein (Cat:GMP-10584-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL21 Receptor Protein (His & AVI Tag), Biotinylated (Cat:11483-H49H-B),The EC₅₀ is 30-90 ng/mL.

蛋白构建

A DNA sequence encoding the Human IL21R (Q9HBE5-1)(Met1-Pro236) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Accession#

Q9HBE5-1

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Cys 20

分子量

The recombinant Human IL21R consists of 243 amino acids and predicts a molecular mass of 28.27 kDa. It migrates as an approximately 50.0 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA.

Recombinant Human IL-21 Receptor/IL-21R Protein (His & AVI Tag), Biotinylated, HPLC-verified: 别称

CD360 Protein, Human; IMD56 Protein, Human; NILR Protein, Human; UNQ3121/PRO10273 Protein, Human

IL-21 Receptor/IL-21R 背景信息

Interleukin-21 receptor, also known as IL-21 receptor, IL-21R, Novel interleukin receptor, IL21R, and NILR, is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and Type 4 subfamily. Interleukin-21 (IL-21) belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL-21R) and the common cytokine receptor gamma chain ( gamma(C) ). The IL-21R is discovered as a novel member of the class-I-cytokine-receptor family and is selectively expressed in lymphoid tissues. IL-21R shows strong sequence homologies to the interleukin-4 receptor alpha chain gene (IL-4RA). The WSXWS motif of IL-21R appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of IL-21R is required for JAK interaction and/or activation. The IL-21R is widely distributed on lymphohematopoietic cells and IL21 impacts some cell types, including CD8+ memory T cells, NK cells, and subsets of CD4 memory T cells. Increased IL21 production is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathological features of autoimmune disease. The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterized by the overproduction of pathogenic autoantibodies.

全称

interleukin 21 receptor

相关通路图

AKT Signaling Pathway

Jak-Stat Signaling Pathway

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Common G-Chain signaling pathway

参考文献

Asao H, et al. (2001) Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 167(1): 1-5.
Wu Z, et al. (2005) Interleukin-21 receptor gene induction in human T cells is mediated by T-cell receptor-induced Sp1 activity. Mol Cell Biol. 25(22): 9741-52.
De Totero D, et al. (2006) Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells. Blood. 107(9): 3708-15.