表达区间及表达系统（Source）
Recombinant Human LY6E Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.
It contains Leu21-Ser101 [Accession | Q16553].
分子量大小（Molecular Weight）
The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.
纯度（Purity）
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
内毒素（Endotoxin）
Less than 1 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
存储（Storage）
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human LY6E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LY6E is greater than 95% as determined by SEC-HPLC.
Immobilized Human LY6E, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6 ng/ml determined by ELISA. (QC Test)
Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety.
Ly-6E; RIG-E; SCA-2; TSA-1; 9804; RIGE; SCA2; TSA1
Yeom CJ, Zeng L, Goto Y, Morinibu A, Zhu Y, Shinomiya K, Kobayashi M, Itasaka S, Yoshimura M, Hur CG, Kakeya H, Hammond EM, Hiraoka M, Harada H. LY6E: a conductor of malignant tumor growth through modulation of the PTEN/PI3K/Akt/HIF-1 axis. Oncotarget. 2016 Oct 4;7(40):65837-65848. doi: 10.18632/oncotarget.11670. PMID: 27589564; PMCID: PMC5323196.
